Micro Cell Culture Technique for the Detection of Neutralization Antibody against Clostridium perfringens Enterotoxin and Its Application to Sero-epidemiology
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چکیده
منابع مشابه
Antibody against Clostridium perfringens type A enterotoxin in human sera.
Antibody against Clostridium perfringens type A enterotoxin was found in 82% of Brazilian and 65% of American serum samples.
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A rapid assay which measured the biological activity of Clostridium perfringens enterotoxin was developed. The method involved the rapid killing of Vero cells by enterotoxin produced by C. perfringens grown in Duncan and Strong sporulation medium. Serial dilutions of toxin were added to Vero cells either in suspension or grown as monolayers in wells of a 96-well cell tissue culture cluster plat...
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Sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative estimation of Clostridium perfringens enterotoxin (CPE) with monoclonal and polyclonal antibodies as capturing and detecting antibodies, respectively. The dose-dependent relationship between absorbance at 405 nm and concentration of purified CPE was obtained over the range of 0.64-400 ng/ml. The sandwich ELISA...
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like any other learning activity, translation is a problem solving activity which involves executing parallel cognitive processes. the ability to think about these higher processes, plan, organize, monitor and evaluate the most influential executive cognitive processes is what flavell (1975) called “metacognition” which encompasses raising awareness of mental processes as well as using effectiv...
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO CLOSTRIDIUM PERFRINGENS ENTEROTOXIN DERIVED FROM DIFFERENT ANIMAL SPECIES
The enterotoxins (ETs) of Clostridium perfringens isolated from enterotoxemic or diarrheic alpacas, pigs, calves, dogs, and horses were obtained from sporulated cell extracts. The ETs from alpaca, pig, and calf isolates were chromatographed on Sephadex G-lOO. Monoclonal antibodies (MAbs) against ETs derived from alpaca, pig, and calf C. perfringens isolates were produced. The MAbs were used...
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ژورنال
عنوان ژورنال: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
سال: 1978
ISSN: 0015-6426,1882-1006
DOI: 10.3358/shokueishi.19.294